Large samples (up to 7 μl) of interstitial fluid were collected from inner media but they contained no measurable LDL the levels of α 2-macroglobulin and albumin were respectively 11 and 18% of plasma concentration. The patients covered the age range 31-96 years relative concentrations of LDL, α 2-macroglobulin and albumin increased in parallel by about 10% per decade. Concentrations were not influenced by depth within the intima, or by distance down the aorta. In interstitial fluid obtained from normal intima of 20 men and women, the mean levels of the proteins were LDL 215%, α 2-macroglobulin 115% and albumin 54% of the concentration in the patient’s own plasma. After equilibration the papers were removed and weighed to measure the amount of interstitial fluid collected, then analysed by quantitative immunoelectrophoresis for three plasma proteins covering a range of molecular masses ( M r): low density lipoprotein (LDL) with molecular mass 2.4 x 10 6, α 2-macroglobulin ( M r 720 000) and serum albumin ( M r 68 000). The interstitial fluid was absorbed onto small, preweighed pieces of filter paper which were inserted into natural strip planes in the tunica intima and inner tunica media. The concentration of plasma proteins was examined in interstitial fluid collected from human aortas, obtained at autopsy, from patients from whom a blood sample had been taken for routine analysis shortly before death.
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